Input format: abi
Reads the ABI "Sanger" capillary sequence traces files, including the PHRED quality scores for the base calls. This allows ABI to FASTQ conversion. Note each ABI file contains one and only one sequence (so there is no point in indexing the file).
Output format: fastq
FASTQ files are a bit like FASTA files but also include sequencing qualities. In Biopython, 'fastq' refers to Sanger style FASTQ files which encode PHRED qualities using an ASCII offset of 33. See also the incompatible 'fastq-solexa' and 'fastq-illumina' variants.
How to convert from abi to fastq ?
You can also convert between these formats by using command line tools.
On Windows install WSL, on
Mac
or Linux start
terminal
Install BioPython
Run following script:
Or you can use this site as online abi to fastq converter by selecting your formats &
file.
Sequence Converter Home page
from Bio import SeqIO
records = SeqIO.parse("THIS_IS_YOUR_INPUT_FILE.abi", "abi")
count = SeqIO.write(records, "THIS_IS_YOUR_OUTPUT_FILE.fastq", "fastq")
print("Converted %i records" % count)