Abi to Fastq Sequence Converter

Input format: abi Reads the ABI "Sanger" capillary sequence traces files, including the PHRED quality scores for the base calls. This allows ABI to FASTQ conversion. Note each ABI file contains one and only one sequence (so there is no point in indexing the file).

Output format: fastq FASTQ files are a bit like FASTA files but also include sequencing qualities. In Biopython, 'fastq' refers to Sanger style FASTQ files which encode PHRED qualities using an ASCII offset of 33. See also the incompatible 'fastq-solexa' and 'fastq-illumina' variants.

You can also convert between these formats by using command line tools.

On Windows install WSL, on Mac or Linux start terminal

Install BioPython

Run following script:

from Bio import SeqIO

records = SeqIO.parse("THIS_IS_YOUR_INPUT_FILE.abi", "abi")
count = SeqIO.write(records, "THIS_IS_YOUR_OUTPUT_FILE.fastq", "fastq")
print("Converted %i records" % count)

Or you can use this site as online abi to fastq converter by selecting your formats & file.