Ace to Fastq Sequence Converter

Input format: ace Reads the contig sequences from an ACE assembly file. Uses Bio.Sequencing.Ace internally clustal The alignment format of Clustal X and Clustal W. See also the Bio.Clustalw module.

Output format: fastq FASTQ files are a bit like FASTA files but also include sequencing qualities. In Biopython, 'fastq' refers to Sanger style FASTQ files which encode PHRED qualities using an ASCII offset of 33. See also the incompatible 'fastq-solexa' and 'fastq-illumina' variants.

You can also convert between these formats by using command line tools.

On Windows install WSL, on Mac or Linux start terminal

Install BioPython

Run following script:

from Bio import SeqIO

records = SeqIO.parse("THIS_IS_YOUR_INPUT_FILE.ace", "ace")
count = SeqIO.write(records, "THIS_IS_YOUR_OUTPUT_FILE.fastq", "fastq")
print("Converted %i records" % count)

Or you can use this site as online ace to fastq converter by selecting your formats & file.