Fasta to Abi Sequence Converter

Input format: fasta This refers to the input FASTA file format introduced for Bill Pearson's FASTA tool, where each record starts with a '>' line. Resulting sequences have a generic alphabet by default.

Output format: abi Reads the ABI "Sanger" capillary sequence traces files, including the PHRED quality scores for the base calls. This allows ABI to FASTQ conversion. Note each ABI file contains one and only one sequence (so there is no point in indexing the file).

You can also convert between these formats by using command line tools.

On Windows install WSL, on Mac or Linux start terminal

Install BioPython

Run following script:

from Bio import SeqIO

records = SeqIO.parse("THIS_IS_YOUR_INPUT_FILE.fasta", "fasta")
count = SeqIO.write(records, "THIS_IS_YOUR_OUTPUT_FILE.abi", "abi")
print("Converted %i records" % count)

Or you can use this site as online fasta to abi converter by selecting your formats & file.