Tab to Fastq-illumina Sequence Converter

Input format: tab Simple two column tab separated sequence files, where each line holds a record's identifier and sequence. For example, this is used by Aligent's eArray software when saving microarray probes in a minimal tab delimited text file.

Output format: fastq-illumina FASTQ files are a bit like FASTA files but also include sequencing qualities. In Biopython, 'fastq' refers to Sanger style FASTQ files which encode PHRED qualities using an ASCII offset of 33. See also the incompatible 'fastq-solexa' and 'fastq-illumina' variants.

You can also convert between these formats by using command line tools.

On Windows install WSL, on Mac or Linux start terminal

Install BioPython

Run following script:

from Bio import SeqIO

records = SeqIO.parse("THIS_IS_YOUR_INPUT_FILE.tab", "tab")
count = SeqIO.write(records, "THIS_IS_YOUR_OUTPUT_FILE.fastq-illumina", "fastq-illumina")
print("Converted %i records" % count)

Or you can use this site as online tab to fastq-illumina converter by selecting your formats & file.