Convert ABI/AB1 sequencing traces to FASTA base calls.

ABI / AB1 trace → FASTA

  • The called sequence is extracted from the trace; chromatogram data and peak information are not included.

Maximum file size: 50 MB. The file is checked before conversion.

About this conversion

The converter reads records in the input format and writes them in the output format. Data that the output format cannot store may be lost. The form above explains the important limits for this pair.

Run it with Biopython

For batch work, you can use Biopython's SeqIO.convert function. If your output needs a sequence type, provide the matching molecule type.

from Bio import SeqIO

count = SeqIO.convert("input.abi", "abi",
                      "output.fasta", "fasta")
print("Converted %i records" % count)

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